Antigen Receptor Specificity and Cell Location Influence the Diversification and Selection of the B-1a Cell Pool with Age.

ABSTRACT: B-1a cells provide immediate and essential protection from infection through production of natural Ig, which is germline-like due to minimal insertion of N region additions. We have previously demonstrated peritoneal B-1a cell-derived phosphorylcholine-specific and total IgM moves away from germline (as evidenced by an increase in N-additions) with age as a result of selection. In young mice, anti-phosphatidylcholine Abs, like anti-phosphorylcholine Abs, contain few N-additions, and have been shown to be essential in protection from bacterial sepsis. In this study, we demonstrate the germline-like status of phosphatidylcholine (PtC)-specific (PtC⁺) peritoneal B-1a cell IgM does not change with age. In direct contrast, the splenic PtC⁺ B-1a cell population does not pre

SUBMITTER: Tsuji N 

PROVIDER: S-EPMC7507122 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature