Long-term in vitro persistence of magnetic properties after magnetic bead-based cell separation of T cells.

ABSTRACT: Magnetic-activated cell sorting (MACS) using magnetic nanoparticles coated with specific antibodies is commonly used in immunology research. For in vitro isolation purposes, it is important to know to what extent the magnetic properties remain present in the isolated cell populations and whether it has consequences for sequential isolations. We hypothesized that only upon cell division, cells will lose their magnetic properties via dilution of the particles in/on their daughter cells. We analysed residual magnetic properties of cells that divided vs cells that did not divide after magnetic bead-based cell separation. As a model, we isolated T cells using beads targeting the non-modulating surface molecule CD45RO. Cells were labelled with the cell division tracking dye PKH and cultured unde

SUBMITTER: Laghmouchi A 

PROVIDER: S-EPMC7507180 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature