Transfection of glycoprotein encoding mRNA for swift evaluation of N-glycan engineering strategies.

ABSTRACT: N-glycosylation is defined as a key quality attribute for the majority of complex biological therapeutics. Despite many N-glycan engineering efforts, the demand to generate desired N-glycan profiles that may vary for different proteins in a reproducible manner is still difficult to fulfill in many cases. Stable production of homogenous structures with a more demanding level of processing, for instance high degrees of branching and terminal sialylation, is particularly challenging. Among many other influential factors, the level of productivity can steer N-glycosylation towards less mature N-glycan structures. Recently, we introduced an mRNA transfection system capable of elucidating bottlenecks in the secretory pathway by stepwise increase of intracellular model protein mRNA load. Here, th

SUBMITTER: Bydlinski N 

PROVIDER: S-EPMC7507192 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature