XBP1 negatively regulates CENPF expression via recruiting ATF6α to the promoter during ER stress.

ABSTRACT:

Background

Centromere protein F (CENPF) is a key component of the kinetochore complex involved in mitosis, cell differentiation and cellular response to stresses. However, the alteration of CENPF in response to endoplasmic reticulum (ER) stress has not been well described. In the present study, we investigate CENPF regulation in response to ER stress.

Methods

Quantitative real-time polymerase chain reaction and western blotting were used to determine CENPF expression under ER stress. Luciferase activity analysis was performed to investigate the promoter regions contributing to CENPF transcription in response to TG. Chromatin immunoprecipitation (ChIP) and ChIP Re-IP assays were used to determine if X-box binding protein 1 (XBP1) and/or activating transcription factor 6α (ATF

SUBMITTER: Shen T 

PROVIDER: S-EPMC7507253 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature