NUP98-HOXA10hd fusion protein sustains multi-lineage haematopoiesis of lineage-committed progenitors in transplant setting.

ABSTRACT:

Objectives

Exploring approaches of extending the haematopoiesis time window of MPPs and lineage-committed progenitors might produce promising therapeutic effects. NUP98-HOXA10hd (NA) fusion protein can expand long-term haematopoietic stem cells (HSCs) and promote engraftment competitiveness without causing obvious oncogenesis. Our objectives were to investigate the roles of NA fusion protein in MPP and downstream lineage-committed progenitor context.

Material and methods

300 sorted MPPs (Lin^- CD48^- c-kit⁺ Sca1⁺ CD135⁺ CD150^- ) were mixed with 5 × 10⁵ total BM helper/competitor cells and injected into irradiated recipients. For secondary transplantation, 5 × 10⁶ total BM cells from primary

SUBMITTER: Dong Y 

PROVIDER: S-EPMC7507399 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature