Parallel Murine and Human Plaque Proteomics Reveals Pathways of Plaque Rupture.

ABSTRACT:

Rationale

Plaque rupture is the proximate cause of most myocardial infarctions and many strokes. However, the molecular mechanisms that precipitate plaque rupture are unknown.

Objective

By applying proteomic and bioinformatic approaches in mouse models of protease-induced plaque rupture and in ruptured human plaques, we aimed to illuminate biochemical pathways through which proteolysis causes plaque rupture and identify substrates that are cleaved in ruptured plaques.

Methods and results

We performed shotgun proteomics analyses of aortas of transgenic mice with macrophage-specific overexpression of urokinase (SR-uPA^+/0 mice) and of SR-uPA^+/0 bone marrow transplant recipients, and we used bioinformatic tools to evaluate protein abundance and funct

SUBMITTER: Vaisar T 

PROVIDER: S-EPMC7508285 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature