Strong Environment-Genotype Interactions Determine the Fitness Costs of Antibiotic Resistance <i>In Vitro</i> and in an Insect Model of Infection.

ABSTRACT: The acquisition of antibiotic resistance commonly imposes fitness costs, a reduction in the fitness of bacteria in the absence of drugs. These costs have been quantified primarily using in vitro experiments and a small number of in vivo studies in mice, and it is commonly assumed that these diverse methods are consistent. Here, we used an insect model of infection to compare the fitness costs of antibiotic resistance in vivo to those in vitro Experiments explored diverse mechanisms of resistance in a Gram-positive pathogen, Bacillus thuringiensis, and a Gram-negative intestinal symbiont, Enterobacter cloacae Rifampin resistance in B. thuringiensis showed fitness costs that were typically elevated in vivo, although these were modulated

SUBMITTER: Manktelow CJ 

PROVIDER: S-EPMC7508608 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature