Developing a broad-range promoter set for metabolic engineering in the thermotolerant yeast Kluyveromyces marxianus.

ABSTRACT: Kluyveromyces marxianus is an emerging host for metabolic engineering. This thermotolerant yeast is the fastest growing eukaryote, has high flux through the TCA cycle, and can metabolize a broad range of C5, C6, and C12 carbon sources. In comparison to the common host Saccharomyces cerevisiae, this non-conventional yeast suffers from a lack of metabolic engineering tools to control gene expression over a wide transcriptional range. To address this issue, we designed a library of 25 native-derived promoters from K. marxanius CBS6556 that spans 87-fold transcriptional strength under glucose metabolism. Six promoters from the library were further characterized in both glucose and xylose as well as across various temperatures from 30 to 45 ​°C. The temperature study reveal

SUBMITTER: Lang X 

PROVIDER: S-EPMC7508702 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature