Localization of the lens intermediate filament switch by imaging mass spectrometry.

ABSTRACT: Imaging mass spectrometry (IMS) enables targeted and untargeted visualization of the spatial localization of molecules in tissues with great specificity. The lens is a unique tissue that contains fiber cells corresponding to various stages of differentiation that are packed in a highly spatial order. The application of IMS to lens tissue localizes molecular features that are spatially related to the fiber cell organization. Such spatially resolved molecular information assists our understanding of lens structure and physiology; however, protein IMS studies are typically limited to abundant, soluble, low molecular weight proteins. In this study, a method was developed for imaging low solubility cytoskeletal proteins in the lens; a tissue that is filled with high concentrations of soluble cr

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7508834 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature