Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity.

ABSTRACT: Evidence of the involvement of epigenetics in pathologies such as cancer, diabetes, and neurodegeneration has increased global interest in epigenetic modifications. For nearly thirty years, it has been known that cancer cells exhibit abnormal DNA methylation patterns. In contrast, the large-scale analysis of histone post-translational modifications (hPTMs) has lagged behind because classically, histone modification analysis has relied on site specific antibody-based techniques. Mass spectrometry (MS) is a technique that holds the promise to picture the histone code comprehensively in a single experiment. Therefore, we developed an MS-based method that is capable of tracking all possible hPTMs in an untargeted approach. In this way, trends in single and combinatorial hPTMs can be reported a

SUBMITTER: Verhelst S 

PROVIDER: S-EPMC7508989 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature