Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation.

ABSTRACT: In the last decade, organoid technology has developed as a primary research tool in basic biological and clinical research. The reliance on poorly defined animal-derived extracellular matrix, however, severely limits its application in regenerative and translational medicine. Here, a well-defined, synthetic biomimetic matrix based on polyisocyanide (PIC) hydrogels that support efficient and reproducible formation of mammary gland organoids (MGOs) in vitro is presented. Only decorated with the adhesive peptide RGD for cell binding, PIC hydrogels allow MGO formation from mammary fragments or from purified single mammary epithelial cells. The cystic organoids maintain their capacity to branch for over two months, which is a fundamental and complex feature during mammary gland development. It

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7509700 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature