VEGF-B Is an Autocrine Gliotrophic Factor for Muller Cells under Pathologic Conditions.

ABSTRACT:

Purpose

Müller glia are important in retinal health and disease and are a major source of retinal VEGF-A. Of the different VEGF family members, the role of VEGF-A in retinal health and disease has been studied extensively. The potential contribution of other VEGF family members to retinal pathophysiology, however, remains poorly defined. This study aimed to understand the role of VEGF-B in Müller cell pathophysiology.

Methods

The expression of different VEGFs and their receptors in human MIO-M1 and mouse QMMuC-1 Müller cell lines and primary murine Müller cells was examined by RT-PCR, ELISA, and Western blot. The effect of recombinant VEGF-B or VEGF-B neutralization on Müller cell viability and survival under normal, hypoxic, and oxidative (4-hydroxynonenal [4-HNE]) conditio

SUBMITTER: Llorian-Salvador M 

PROVIDER: S-EPMC7509798 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature