Derivation and propagation of spermatogonial stem cells from human pluripotent cells.

ABSTRACT:

Objectives

This study is designed to generate and propagate human spermatogonial stem cells (SSCs) derived from human pluripotent stem cells (hPSCs).

Methods

hPSCs were differentiated into SSC-like cells (SSCLCs) by a three-step strategy. The biological characteristics of SSCLCs were detected by immunostaining with antibodies against SSC markers. The ability of self-renewal was measured by propagating for a long time and still maintaining SSCs morphological property. The differentiation potential of SSCLCs was determined by the generation of spermatocytes and haploid cells, which were identified by immunostaining and flow cytometry. The transcriptome analysis of SSCLCs was performed by RNA sequencing. The biological function of SSCLCs was assessed by xeno-transplantation int

SUBMITTER: Xu H 

PROVIDER: S-EPMC7509941 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature