Stepwise differentiation and functional characterization of human induced pluripotent stem cell-derived choroidal endothelial cells.

ABSTRACT:

Background

Endothelial cells (ECs) are essential regulators of the vasculature, lining arteries, veins, and capillary beds. While all ECs share a number of structural and molecular features, heterogeneity exists depending on their resident tissue. ECs lining the choriocapillaris in the human eye are lost early in the pathogenesis of age-related macular degeneration (AMD), a common and devastating form of vision loss. In order to study the mechanisms leading to choroidal endothelial cell (CEC) loss and to develop reagents for repairing the choroid, a reproducible in vitro model, which closely mimic CECs, is needed. While a number of protocols have been published to direct induced pluripotent stem cells (iPSCs) into ECs, the goal of this study was to develop methods to differentiate

SUBMITTER: Mulfaul K 

PROVIDER: S-EPMC7510078 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature