Somatic mutation detection efficiency in EGFR: a comparison between high resolution melting analysis and Sanger sequencing.

ABSTRACT:

Background

High resolution melting curve analysis is a cost-effective rapid screening method for detection of somatic gene mutation. The performance characteristics of this technique has been explored previously, however, analytical parameters such as limit of detection of mutant allele fraction and total concentration of DNA, have not been addressed. The current study focuses on comparing the mutation detection efficiency of High-Resolution Melt Analysis (HRM) with Sanger Sequencing in somatic mutations of the EGFR gene in non-small cell lung cancer.

Methods

The minor allele fraction of somatic mutations was titrated against total DNA concentration using Sanger sequencing and HRM to determine the limit of detection. The mutant and wildtype allele fractions were validated by

SUBMITTER: Joy RA 

PROVIDER: S-EPMC7510081 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature