ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems.

ABSTRACT:

Background

Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus. Current cytosine or adenine base editors can only accomplish C-to-T (G-to-A) or A-to-G (T-to-C) substitutions in the windows of target genomic sites of organisms; therefore, there is a need to develop base editors that can simultaneously achieve C-to-T and A-to-G substitutions at the targeting site.

Results

In this study, a novel fusion adenine and cytosine base editor (ACBE) was generated by fusing a heterodimer of TadA (ecTadA^WT/*) and an activation-induced cytidine deaminase (AID) to the N- and C-terminals of Cas9 nickase (nCas9), respectively.

SUBMITTER: Xie J 

PROVIDER: S-EPMC7510086 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature