Site-specific opening of the blood-brain barrier by extracellular histones.

ABSTRACT:

Background

Increased extracellular histones in the bloodstream are known as a biomarker for vascular dysfunction associated with severe trauma or sepsis. There is limited information regarding the pathogenic role of circulating histones in neuroinflammation and cerebrovascular endothelial injury. Particularly, it remains unclear whether histones affect the blood-brain barrier (BBB) permeability function.

Methods

The direct effects of unfractionated histones on endothelial barrier properties were first assessed in brain microvascular endothelial cell monolayers by measuring transendothelial electrical resistance and solute flux. This was followed by in vivo mouse experiments, where BBB function was assessed by quantifying brain tissue accumulation of intravenously injected tr

SUBMITTER: Villalba N 

PROVIDER: S-EPMC7510151 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature