Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts.

ABSTRACT: Hydrogel-based three-dimensional (3D) cellular models are attractive for bioengineering and pharmaceutical development as they can more closely resemble the cellular function of native tissue outside of the body. In general, these models are composed of tissue specific cells embedded within a support material, such as a hydrogel. As hydrogel properties directly affect cell function, hydrogel composition is often tailored to the cell type(s) of interest and the functional objective of the model. Here, we develop a parametric analysis and screening method to identify suitable encapsulation conditions for the formation of myotubes from primary murine myoblasts in methacryloyl gelatin (GelMA) hydrogels. The effect of the matrix properties on the myotube formation was investigated by varying Ge

SUBMITTER: Deshmukh DV 

PROVIDER: S-EPMC7510461 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature