Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus.

ABSTRACT: Bacillus methanolicus MGA3 is a thermotolerant and relatively fast-growing methylotroph able to secrete large quantities of glutamate and lysine. These natural characteristics make B. methanolicus a good candidate to become a new industrial chassis organism, especially in a methanol-based economy. Intriguingly, the only substrates known to support B. methanolicus growth as sole sources of carbon and energy are methanol, mannitol, and, to a lesser extent, glucose and arabitol. Because fluxomics provides the most direct readout of the cellular phenotype, we hypothesized that comparing methylotrophic and nonmethylotrophic metabolic states at the flux level would yield new insights into MGA3 metabolism. In this study, we designed and performed a ¹³C metabolic fl

SUBMITTER: Delepine B 

PROVIDER: S-EPMC7511216 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature