Ancestral sequence reconstruction produces thermally stable enzymes with mesophilic enzyme-like catalytic properties.

ABSTRACT: Enzymes have high catalytic efficiency and low environmental impact, and are therefore potentially useful tools for various industrial processes. Crucially, however, natural enzymes do not always have the properties required for specific processes. It may be necessary, therefore, to design, engineer, and evolve enzymes with properties that are not found in natural enzymes. In particular, the creation of enzymes that are thermally stable and catalytically active at low temperature is desirable for processes involving both high and low temperatures. In the current study, we designed two ancestral sequences of 3-isopropylmalate dehydrogenase by an ancestral sequence reconstruction technique based on a phylogenetic analysis of extant homologous amino acid sequences. Genes encoding the designed

SUBMITTER: Furukawa R 

PROVIDER: S-EPMC7511310 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature