Modulation of CYP2C9 activity and hydrogen peroxide production by cytochrome b₅.

ABSTRACT: Cytochromes P450 (CYP) play a major role in drug detoxification, and cytochrome b₅ (cyt b5) stimulates the catalytic cycle of mono-oxygenation and detoxification reactions. Collateral reactions of this catalytic cycle can lead to a significant production of toxic reactive oxygen species (ROS). One of the most abundant CYP isoforms in the human liver is CYP2C9, which catalyzes the metabolic degradation of several drugs including nonsteroidal anti-inflammatory drugs. We studied modulation by microsomal membrane-bound and soluble cyt b5 of the hydroxylation of salicylic acid to gentisic acid and ROS release by CYP2C9 activity in human liver microsomes (HLMs) and by CYP2C9 baculosomes. CYP2C9 accounts for nearly 75% of salicylic acid hydroxylation in HLMs at concentrations reached a

SUBMITTER: Gomez-Tabales J 

PROVIDER: S-EPMC7511354 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature