Automated AFM analysis of DNA bending reveals initial lesion sensing strategies of DNA glycosylases.

ABSTRACT: Base excision repair is the dominant DNA repair pathway of chemical modifications such as deamination, oxidation, or alkylation of DNA bases, which endanger genome integrity due to their high mutagenic potential. Detection and excision of these base lesions is achieved by DNA glycosylases. To investigate the remarkably high efficiency in target site search and recognition by these enzymes, we applied single molecule atomic force microscopy (AFM) imaging to a range of glycosylases with structurally different target lesions. Using a novel, automated, unbiased, high-throughput analysis approach, we were able to resolve subtly different conformational states of these glycosylases during DNA lesion search. Our results lend support to a model of enhanced lesion search efficiency through initial

SUBMITTER: Bangalore DM 

PROVIDER: S-EPMC7511397 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature