Racemic phosphorothioate as a tool for NMR investigations of protein-DNA complexes.

ABSTRACT: A major driving force for protein-nucleic acid association is electrostatic interactions via ion pairs of the positively charged basic side chains and negatively charged phosphates. For a better understanding of how proteins scan DNA and recognize particular signatures, it is important to gain atomic-level insight into the behavior of basic side chains at the protein-DNA interfaces. NMR spectroscopy is a powerful tool for investigating the structural, dynamic, and kinetic aspects of protein-DNA interactions. However, resonance assignment of basic side-chain cationic moieties at the molecular interfaces remains to be a major challenge. Here, we propose a fast, robust, and inexpensive approach that greatly facilitates resonance assignment of interfacial moieties and also allows for kinetic m

SUBMITTER: Nepravishta R 

PROVIDER: S-EPMC7511421 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature