Targeted delivery of miR-99b reprograms tumor-associated macrophage phenotype leading to tumor regression.

ABSTRACT:

Background

Accumulating evidence has shown that tumor-associated macrophages (TAMs) play a critical role in tumor progression. Targeting TAMs is a potential strategy for tumor immunotherapy. However, the mechanism underlying the TAM phenotype and function needs to be resolved. Our previous studies have demonstrated that miR-125a can reverse the TAM phenotype toward antitumor. Meanwhile, we have found that miR-125a and miR-99b cluster in the first intron of the same host gene, and are transcribed simultaneously in bone marrow-derived macrophages (BMDMs) following LPS+IFNγ stimulation. However, it remains unclear whether miR-99b by itself can exert an antitumor effect by regulating macrophage phenotype.

Methods

miR-99b and/or miR-125a were delivered into TAMs of orthotopic hep

SUBMITTER: Wang L 

PROVIDER: S-EPMC7511616 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature