Expression Landscape of circRNAs in Arabidopsis thaliana Seedlings and Adult Tissues.

ABSTRACT: RNA-seq is currently the only method that can provide a comprehensive landscape of circular RNA (circRNAs) in the whole organism and its particular organs. Recent years have brought an increasing number of RNA-seq-based reports on plant circRNAs. Notably, the picture they revealed is questionable and depends on the applied circRNA identification and quantification techniques. In consequence, little is known about the biogenesis and functions of circRNAs in plants. In this work, we tested two experimental and six bioinformatics procedures of circRNA analysis to determine the optimal approach for studying the profiles of circRNAs in Arabidopsis thaliana. Then using the optimized strategy, we determined the accumulation of circular and corresponding linear transcripts in plant seedling

SUBMITTER: Philips A 

PROVIDER: S-EPMC7511659 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature