Production of superoxide and hydrogen peroxide in the mitochondrial matrix is dominated by site I_Q of complex I in diverse cell lines.

ABSTRACT: Understanding how mitochondria contribute to cellular oxidative stress and drive signaling and disease is critical, but quantitative assessment is difficult. Our previous studies of cultured C2C12 cells used inhibitors of specific sites of superoxide and hydrogen peroxide production to show that mitochondria generate about half of the hydrogen peroxide released by the cells, and site I_Q of respiratory complex I produces up to two thirds of the superoxide and hydrogen peroxide generated in the mitochondrial matrix. Here, we used the same approach to measure the engagement of these sites in seven diverse cell lines to determine whether this pattern is specific to C2C12 cells, or more general. These diverse cell lines covered primary, immortalized, and cancerous cells, from seven t

SUBMITTER: Fang J 

PROVIDER: S-EPMC7511732 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature