The Pseudomonas aeruginosa protease LasB directly activates IL-1β.

ABSTRACT:

Background

Pulmonary damage by Pseudomonas aeruginosa during cystic fibrosis lung infection and ventilator-associated pneumonia is mediated both by pathogen virulence factors and host inflammation. Impaired immune function due to tissue damage and inflammation, coupled with pathogen multidrug resistance, complicates the management of these deep-seated infections. Pathological inflammation during infection is driven by interleukin-1β (IL-1β), but the molecular processes involved are not fully understood.

Methods

We examined IL-1β activation in a pulmonary model infection of Pseudomonas aeruginosa and in vitro using genetics, specific inhibitors, recombinant proteins, and targeted reporters of protease activity and IL-1β bioactivity.

Findings

Caspase-family inflammasome

SUBMITTER: Sun J 

PROVIDER: S-EPMC7511813 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature