Topology of the U12-U6_atac snRNA Complex of the Minor Spliceosome and Binding by NTC-Related Protein RBM22.

ABSTRACT: Splicing of precursor messenger RNA is catalyzed by the spliceosome, a dynamic ribonucleoprotein assembly including five small nuclear (sn)RNAs and >100 proteins. RNA components catalyze the two transesterification reactions, but proteins perform critical roles in assembly and rearrangement. The catalytic core comprises a paired complex of U2 and U6 snRNAs for the major form of the spliceosome and U12 and U6_atac snRNAs for the minor variant (∼0.3% of all spliceosomes in higher eukaryotes); the latter shares key catalytic sequence elements and performs identical chemistry. Here we use solution NMR techniques to show that the U12-U6_atac snRNA complex of both human and Arabidopsis maintain base-pairing patterns similar to those in the three-helix model of the U2-U

SUBMITTER: Ciavarella J 

PROVIDER: S-EPMC7512442 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature