Molecular identification and evolutionary relationships between the subspecies of Musa by DNA barcodes.

ABSTRACT:

Background

The banana (Musa sp., AAA) genome is constantly increasing due to high-frequency of somaclonal variations. Due to its large diversity, a conventional numerical and morphological based taxonomic identification of banana cultivars is laborious, difficult and often leads to subject of disagreements.

Results

Hence, in the present study, we used universal DNA barcode ITS2 region to identify and to find the genetic relationship between the cultivars and varieties of banana. Herein, a total of 16 banana cultivars were PCR amplified using ITS2 primer pair. In addition, 321 sequences which were retrieved from GenBank, USA, were used in this study. The sequences were then aligned using Clustal W and genetic distances were computed using MEGA V5.1. The study showed significa

SUBMITTER: Dhivya S 

PROVIDER: S-EPMC7513480 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature