Interferon-beta enhances sensitivity to gemcitabine in pancreatic cancer.

ABSTRACT:

Background

Adjuvant gemcitabine for pancreatic cancer has limited efficacy in the clinical setting. Impaired drug metabolism is associated with treatment resistance. We aimed to evaluate the chemosensitising effect of interferon-beta (IFN-β).

Methods

BxPC-3, CFPAC-1, and Panc-1 cells were pre-treated with IFN-β followed by gemcitabine monotherapy. The effect on cell growth, colony formation, and cell cycle was determined. RT-qPCR was used to measure gene expression. BxPC-3 cells were used in a heterotopic subcutaneous mouse model.

Results

IFN-β increased sensitivity to gemcitabine (4-, 7.7-, and 1.7-fold EC₅₀ decrease in BxPC-3, CFPAC-1, and Panc-1, respectively; all P < 0.001). Findings were confirmed when assessing colony formation. The percentage of cell

SUBMITTER: Blaauboer A 

PROVIDER: S-EPMC7513525 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature