Positive-feedback, ratiometric biosensor expression improves high-throughput metabolite-producer screening efficiency in yeast.

ABSTRACT: Biosensors are valuable and versatile tools in synthetic biology that are used to modulate gene expression in response to a wide range of stimuli. Ligand responsive transcription factors are a class of biosensor that can be used to couple intracellular metabolite concentration with gene expression to enable dynamic regulation and high-throughput metabolite producer screening. We have established the Saccharomyces cerevisiae WAR1 transcriptional regulator and PDR12 promoter as an organic acid biosensor that can be used to detect varying levels of para-hydroxybenzoic acid (PHBA) production from the shikimate pathway and output green fluorescent protein (GFP) expression in response. The dynamic range of GFP expression in response to PHBA was dramatically increased by engineering

SUBMITTER: Williams TC 

PROVIDER: S-EPMC7513737 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature