Central nervous system and peripheral cell labeling by vascular endothelial cadherin-driven lineage tracing in adult mice.

ABSTRACT: Understanding the contribution of endothelial cells to the progenitor pools of adult tissues has the potential to inform therapies for human disease. To address whether endothelial cells transdifferentiate into non-vascular cell types, we performed cell lineage tracing analysis using transgenic mice engineered to express a fluorescent marker following activation by tamoxifen in vascular endothelial cadherin promoter-expressing cells (VEcad-CreERT2; B6 Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze). Activation of target-cell labeling following 1.5 months of ad libitum feeding with tamoxifen-laden chow in 4-5 month-old mice resulted in the tracing of central nervous system and peripheral cells that include: cerebellar granule neurons, ependymal cells, skeletal myocytes, pancreatic beta cells, pancrea

SUBMITTER: Soto-Avellaneda A 

PROVIDER: S-EPMC7513977 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature