The effect of MD1 on potassium and L-type calcium current of cardiomyocytes from high-fat diet mice.

ABSTRACT: Myeloid differentiation protein 1 (MD1) is exerted an anti-arrhythmic effect in obese mice. Therefore, we sought to clarify whether MD1 can alter the electrophysiological remodeling of cardiac myocytes from obese mice by regulating voltage-gated potassium current and calcium current. MD1 knock-out (KO) and wild type (WT) mice were given a high-fat diet (HFD) for 20 weeks, starting at the age of 6 weeks. The potential electrophysiological mechanisms were estimated by whole-cell patch-clamp and molecular analysis. After 20-week HFD feeding, action potential duration (APD) from left ventricular myocytes of MD1-KO mice revealed APD₂₀, APD₅₀, and APD₉₀ were profoundly enlarged. Furthermore, HFD mice showed a decrease in the fast transient outward potassium curre

SUBMITTER: Shuai W 

PROVIDER: S-EPMC7515570 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature