Engineered signal-coupled inducible promoters: measuring the apparent RNA-polymerase resource budget.

ABSTRACT: Inducible promoters are a central regulatory component in synthetic biology, metabolic engineering, and protein production for laboratory and commercial uses. Many of these applications utilize two or more exogenous promoters, imposing a currently unquantifiable metabolic burden on the living system. Here, we engineered a collection of inducible promoters (regulated by LacI-based transcription factors) that maximize the free-state of endogenous RNA polymerase (RNAP). We leveraged this collection of inducible promotors to construct simple two-channel logical controls that enabled us to measure metabolic burden - as it relates to RNAP resource partitioning. The two-channel genetic circuits utilized sets of signal-coupled transcription factors that regulate cognate inducible promoters in a co

SUBMITTER: Davey JA 

PROVIDER: S-EPMC7515704 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature