Native de novo structural determinations of non-canonical nucleic acid motifs by X-ray crystallography at long wavelengths.

ABSTRACT: Obtaining phase information remains a formidable challenge for nucleic acid structure determination. The introduction of an X-ray synchrotron beamline designed to be tunable to long wavelengths at Diamond Light Source has opened the possibility to native de novo structure determinations by the use of intrinsic scattering elements. This provides opportunities to overcome the limitations of introducing modifying nucleotides, often required to derive phasing information. In this paper, we build on established methods to generate new tools for nucleic acid structure determinations. We report on the use of (i) native intrinsic potassium single-wavelength anomalous dispersion methods (K-SAD), (ii) use of anomalous scattering elements integral to the crystallization buffer (extrinsic cobalt and i

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7515729 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature