A novel setup for simultaneous two-photon functional imaging and precise spectral and spatial visual stimulation in Drosophila.

ABSTRACT: Motion vision has been extensively characterised in Drosophila melanogaster, but substantially less is known about how flies process colour, or how spectral information affects other visual modalities. To accurately dissect the components of the early visual system responsible for processing colour, we developed a versatile visual stimulation setup to probe combined spatial, temporal and spectral response properties. Using flies expressing neural activity indicators, we tracked visual responses in the medulla, the second visual neuropil, to a projected colour stimulus. The introduction of custom bandpass optical filters enables simultaneous two-photon imaging and visual stimulation over a large range of wavelengths without compromising the temporal stimulation rate. With monochromator-prod

SUBMITTER: Feord RC 

PROVIDER: S-EPMC7515906 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature