Establishment of a stable transfection method in Babesia microti and identification of a novel bidirectional promoter of Babesia microti.

ABSTRACT: Babesia microti, an emerging human pathogen, is primarily transmitted through a bite of an infected tick and blood transfusions in human. Stable transfection technique has been reported in many protozoan parasites over the past few years. However, in vivo transient and stable transfection method has not been established for Babesia microti. Here, for the first time, we present a method of transient as well as stable transfection of the Babesia microti (B. microti) in the in vivo conditions. We have identified a novel promoter of B. microti. We also demonstrated that Plasmodium berghei DHFR promoter is recognized and functional in B. microti. We show that BM-CTQ41297 promoter control the expression of two genes, which are present on either side and thus represents a bi-functional promoter i

SUBMITTER: Jaijyan DK 

PROVIDER: S-EPMC7515924 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature