Inspired by the human placenta: a novel 3D bioprinted membrane system to create barrier models.

ABSTRACT: Barrier organ models need a scaffold structure to create a two compartment culture. Technical filter membranes used most often as scaffolds may impact cell behaviour and present a barrier themselves, ultimately limiting transferability of test results. In this work we present an alternative for technical filter membrane systems: a 3D bioprinted biological membrane in 24 well format. The biological membrane, based on extracellular matrix (ECM), is highly permeable and presents a natural 3D environment for cell culture. Inspired by the human placenta we established a coculture of a trophoblast-derived cell line (BeWo b30), together with primary placental fibroblasts within the biological membrane (simulating villous stroma) and primary human placental endothelial cells-representing three cel

SUBMITTER: Kreuder AE 

PROVIDER: S-EPMC7515925 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature