Longitudinal mouse-PET imaging: a reliable method for estimating binding parameters without a reference region or blood sampling.

ABSTRACT: Longitudinal mouse PET imaging is becoming increasingly popular due to the large number of transgenic and disease models available but faces challenges. These challenges are related to the small size of the mouse brain and the limited spatial resolution of microPET scanners, along with the small blood volume making arterial blood sampling challenging and impossible for longitudinal studies. The ability to extract an input function directly from the image would be useful for quantification in longitudinal small animal studies where there is no true reference region available such as TSPO imaging.

Methods

Using dynamic, whole-body ¹⁸F-DPA-714 PET scans (60 min) in a mouse model of hippocampal sclerosis, we applied a factor analysis (FA) approach to extract an image-derived

SUBMITTER: Wimberley C 

PROVIDER: S-EPMC7515949 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature