Improving lipid productivity by engineering a control-knob gene in the oleaginous microalga Nannochloropsis oceanica.

ABSTRACT: Nannochloropsis spp. are promising industrial microalgae for scalable oil production and the lipid production can be boosted by nutrient starvation and high irradiance. However, these stimuli halt growth, thereby decreasing overall productivity. In this study, we created transgenic N. oceanica where AtDXS gene encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS) derived from Arabidopsis thaliana was overexpressed in vivo. Compared with the wild type (WT), engineered Nannochloropsis showed a higher CO₂ absorption capacity and produced more biomass, lipids, and carbohydrates with more robust growth in either preferred conditions or various stressed conditions (low light, high light, nitrogen starvation, and trace element depletion). Spec

SUBMITTER: Han X 

PROVIDER: S-EPMC7516279 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature