Neutrophil extracellular traps in stored canine red blood cell units.

ABSTRACT:

Background

Neutrophil extracellular traps (NETs), webs of DNA and citrullinated histones extruded from activated neutrophils cause transfusion-related acute lung injury. Supernatants of stored red blood cell (RBC) units might promote NETosis in neutrophils from the units or from transfusion recipients.

Hypotheses

(1) NETs form during storage of canine RBC, (2) leukoreduction (LR) before storage of RBC reduces NETosis, and (3) supernatant from stored, nonleukoreduced (NLR) RBC units induces NETosis in healthy canine neutrophils modeling transfusion recipients.

Animals

Six healthy purpose-bred research dogs were utilized for blood donation.

Methods

Prospective controlled study. RBC units were collected from each dog, aseptically divided into 2 equal subunits, 1 o

SUBMITTER: McQuinn ER 

PROVIDER: S-EPMC7517510 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature