MiR-138-5p suppresses glioblastoma cell viability and leads to cell cycle arrest by targeting cyclin D3.

ABSTRACT: Although malignant glioblastoma (GBM) treatment has significantly improved in the past few decades, the prognosis of GBM remains unsatisfactory. MicroRNA (miR)-138-5p has been reported as a tumor suppressor in several types of human cancer; however, little is known about the function of miR-138-5p in GBM. The present study aimed to investigate the role of miR-138-5p in GBM as well as the underlying molecular mechanisms. The present study performed bioinformatics analysis, reverse transcription-quantitative (RT-q)PCR, western blotting, cell viability assays, colony formation assays, invasion assays and cell cycle analysis to investigate the biological function of miR-138-5p in both patient tissues and cell lines. In addition, miR-138-5p targets in GBM were predicted using Gene Expression Om

SUBMITTER: Wu H 

PROVIDER: S-EPMC7517571 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature