RNA-Seq identifies condition-specific biological signatures of ischemia-reperfusion injury in the human kidney.

ABSTRACT:

Background

Acute kidney injury (AKI) is defined as a sudden event of kidney failure or kidney damage within a short period. Ischemia-reperfusion injury (IRI) is a critical factor associated with severe AKI and end-stage kidney disease (ESKD). However, the biological mechanisms underlying ischemia and reperfusion are incompletely understood, owing to the complexity of these pathophysiological processes. We aimed to investigate the key biological pathways individually affected by ischemia and reperfusion at the transcriptome level.

Results

We analyzed the steady-state gene expression pattern of human kidney tissues from normal (pre-ischemia), ischemia, and reperfusion conditions using RNA-sequencing. Conventional differential expression and self-organizing map (SOM) clustering

SUBMITTER: Park M 

PROVIDER: S-EPMC7517631 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature