Joint analysis of lncRNA m⁶A methylome and lncRNA/mRNA expression profiles in gastric cancer.

ABSTRACT:

Background

N ⁶-methyladenosine (m⁶A) modification might be closely associated with the genesis and development of gastric cancer (GC). Currently, the evidence established by high-throughput assay for GC-related m⁶A patterns based on long non-coding RNAs (lncRNAs) remains limited. Here, a joint analysis of lncRNA m⁶A methylome and lncRNA/mRNA expression profiles in GC was performed to explore the regulatory roles of m⁶A modification in lncRNAs.

Methods

Three subjects with primary GC were enrolled in our study and paired sample was randomly selected from GC tissue and adjacent normal tissue for each case. Methylated RNA Immunoprecipitation NextGeneration Sequencing (MeRIP-Seq) and Microarray Gene Expression Profilin

SUBMITTER: Lv Z 

PROVIDER: S-EPMC7517696 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature