A Protocol for Cancer-Related Mutation Detection on Exosomal DNA in Clinical Application.

ABSTRACT:

Background

Recently, some genomic mutations in exosomal DNA have been found to be related to disease progress and clinical outcomes of patients in several cancers. Unfortunately, the methods for exosome isolation and exosomal DNA analysis are still lack of relevant research to ensure their optimal performance and the comparability. Here we aim to establish a protocol for cancer-related mutation detection on exosomal DNA in clinical application.

Methods

Taking KRAS mutation in pancreatic cancer as an example, we tested whether the types of blood samples, the potential factors in the courses of exosome isolation and exosomal DNA preparation, as well as the detail in mutation detection by droplet digital PCR (ddPCR) could influence the exosomal DNA analysis.

Results</

SUBMITTER: Wang ZY 

PROVIDER: S-EPMC7518026 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature