Rapid fabrication of sieved microwells and cross-flow microparticle trapping.

ABSTRACT: The use of microwells is popular for a wide range of applications due to its' simplicity. However, the seeding of conventional microwells, which are closed at the bottom, is restricted to gravitational sedimentation for cell or particle deposition and therefore require lengthy settling times to maximize well occupancy. The addition of microfluidics to the capture process has accelerated cell or particle dispersion and improved capture ability but is mostly limited to gravitationally-driven settling for capture into the wells. An alternative approach to conventional closed-microwells, sieved microwells supersedes reliance on gravity by using hydrodynamic forces through the open pores at the bottom of the microwells to draw targets into the wells. We have developed a rapid fabrication method

SUBMITTER: Romita L 

PROVIDER: S-EPMC7518267 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature