Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy.

ABSTRACT: Changes in the elastic properties of living tissues during normal development and in pathological processes are often due to modifications of the collagen component of the extracellular matrix at various length scales. Force volume AFM can precisely capture the mechanical properties of biological samples with force sensitivity and spatial resolution. The integration of AFM data with data of the molecular composition contributes to understanding the interplay between tissue biochemistry, organization and function. The detection of micrometer-size, heterogeneous domains at different elastic moduli in tissue sections by AFM has remained elusive so far, due to the lack of correlations with histological, optical and biochemical assessments. In this work, force volume AFM is used to identify col

SUBMITTER: Calo A 

PROVIDER: S-EPMC7518416 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature