Isolation of extracellular vesicles improves the detection of mutant DNA from plasma of metastatic melanoma patients.

ABSTRACT: Detection of BRAF^V600E within cell free tumor DNA (ctDNA) is emerging as a promising means to improve patients' stratification or enable BRAF inhibitor (BRAFi) therapeutic monitoring in a minimally invasive manner. Here, we investigated whether extracellular vesicle-(EV)-associated-DNA (EV-DNA) has value as an alternative source of circulating BRAF^V600E. To do so, we identified a clinical practice-compatible protocol for the isolation of EV-DNA and assessed BRAF gene status on plasma samples from metastatic melanoma patients at the beginning and during BRAFi therapy. This protocol uses a peptide with high affinity for EVs and it has been found to recover more mutant DNA from plasma than standard ultracentrifugation. Molecular analyses revealed that mutant DNA is large

SUBMITTER: Zocco D 

PROVIDER: S-EPMC7519075 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature