In vivo ³¹P magnetic resonance spectroscopy study of mouse cerebral NAD content and redox state during neurodevelopment.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD) is an important cofactor of energy-producing pathways. The redox ratio (NAD⁺/NADH) reflects the cellular oxidoreductive state. Oxidative stress and redox dysregulation have been suggested to contribute to various neurological diseases. The assessment of NAD content has been recently demonstrated in large animals and human brains by ³¹P magnetic resonance spectroscopy. However, its measurement in small rodents has never been attempted. The purpose of this study was to investigate, in vivo, the NAD content during mouse brain neurodevelopment. ³¹P-MR-spectra were acquired in the mouse brain at postnatal days P20, P40, P90 and P250 at 14.1 T using a 3D-localization sequence. High spectral quality was achieved at 14.1 T. N

SUBMITTER: Skupienski R 

PROVIDER: S-EPMC7519085 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature