Hypothermic oxygenated perfusion protects from mitochondrial injury before liver transplantation.

ABSTRACT:

Background

Mitochondrial succinate accumulation has been suggested as key event for ischemia reperfusion injury in mice. No specific data are however available on behavior of liver mitochondria during ex situ machine perfusion in clinical transplant models.

Methods

We investigated mitochondrial metabolism of isolated perfused rat livers before transplantation. Livers were exposed to warm and cold ischemia to simulate donation after circulatory death (DCD) and organ transport. Subsequently, livers were perfused with oxygenated Belzer-MPS for 1h, at hypothermic or normothermic conditions. Various experiments were performed with supplemented succinate and/or mitochondrial inhibitors. The perfusate, liver tissues, and isolated mitochondria were analyzed by mass-spectroscopy and

SUBMITTER: Schlegel A 

PROVIDER: S-EPMC7519249 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature